Tag Archives: Dr. Katherine Mastrota

You Know What Bugs Me? Fluorescein Quenching… Dr. Katherine Mastrota You know what bugs me?  Fluorescein quenching.  You know, the phenomenon of reduced fluorescent efficiency at high concentrations of fluorescein.  Fluorescein is subject to quenching, or dampening of the emitted signal strength, by various factors. One such factor is self-quenching, in which individual fluorescein molecules [...]

The technique of applying vital dyes to examine the ocular surface (cornea, conjunctiva and lid margin) can influence the information gathered.  Flooding the surface with copious amounts of dye is messy, increases the time needed to analyze the stain, and, most importantly, can overwhelm the surface masking subtle findings.  A very small amount of dye [...]

I once told a class of 1,000 colleagues that lissamine green solution had “changed my life”.  To me, the brilliance of lissamine in solution was far superior to the stain I could achieve with a lissamine strip. Pathology of the ocular surface was easily identifiable and demonstrable to the patient via imaging.  Once satisfied with [...]

It is widely accepted that an abnormal, hyperosmolar tear film cannot successfully support the ocular surface ultimately leading to a loss of mucin-producing goblet cells and an increase in conjunctival and corneal surface desquamation. In general, the cell-to-cell junctions of the corneal epithelium are more resistant to the adverse effects of a dysfunional tear film [...]